Information about the course

  • Level: postbachelor
  • Duration: 3 days
  • Study costs: € 1.725,= (excl. arrangement costs and learning materials)
  • Start date: December 17, 2018
  • Course location: Breda


GC-MS is an important technique for the identification of volatile components. In this course, you will learn to interpret GC-MS mass spectra systematically.

Target group

You work as a (senior) analyst or employee in a bachelor level laboratory and work with GC-MS, for example in environmental laboratories, the food industry, the chemical industry or pharmacy. You want to gain insight into the fragmentation of ions in GC-MS and learn how to obtain information about the molecular structure from mass spectra. You have a good basic knowledge of organic chemistry. Since this is indispensable, it forms a major requirement.

End result

At the end of this course, you are able interpret simple to slightly complex EI mass spectra. You can assign fragment ions of molecules. This helps you to solve confirmation and identification problems in GC-MS analyzes. You are able to critically evaluate the results of library search procedures.


Day 1 (December 17. 2018):

  • Introduction to electron ionization mass spectrometry
  • Molecule ions, (accurate) mass, nitrogen rule, RDBE
  • General fragmentation rules
  • Important fragmentation mechanisms: σ and α cleavage reactions

Day 2 (December 18, 2018):

  • Isotopes
  • Important fragmentation mechanisms: Inductive cleavage reaction, McLafferty rearrangement
  • Development in GC-MS: Low-bleed columns
  • Important fragmentation mechanisms: Ring fragmentation, among others retro-Diels-Alder fragmentation
  • General interpretation procedure, ion series.

Day 3 (December 19, 2018):

  • Use of mass spectral libraries
  • Important fragmentation mechanisms: Secondary fragmentation reactions
  • Mass spectra of component classes
  • Own cases, questions and answers

More information

Here you can find more information about the course.

Do you have any questions about the course or are you interested in an in-company training?
Contact course manager John Hageman via +31 76 525 8888 or mail to